159
using direct fatty acid methylation of samples and
using SupelcoTM SP 2560 column which provided
clear separation of the fatty acids including Cis and
Trans isomers. Low levels of arachidic (0.45 ± 0.07),
behenic (0.19 ± 0.06), lignoceric (0.27 ± 0.12), ner-
vonic (0.07 ± 0.02) and eicosenoic (0.32 ± 0.06) acids
were also observed in all the rice samples studied
here. Most studies on fatty acids composition in rice
(Khatoon and Goapalakrishna, 2004; Mano et al,
1999; Taira and Chang 1986; Yhoshida et al, 2010;
Zhou et al, 2003) failed to detect and report these
minor fatty acids which together accounts for about
1.6% of the total fatty acids. The wide range of individ-
ual fatty acids in the diverse rice varieties shows that
rice breeding can be designed to increase individual
fatty acid of interest for improved health benefits.
Correlations were observed between many combi-
nations of fatty acids is shown in table 6. The major
fatty acid palmitic showed significant correlation
with capric or myristic acids while oleic showed sig-
Figure 3. Fatty acids chromatogram of rice sample
Table 6 .Correction among the fatty acids is high yielding Indian rice varieties
Capric Myristic Palmitic Stearic Arachidic Behenic Lignoceric Palmitoleic Oleic Eicosenoic Nervonic Linoleic
Myristic 0 .65 9
Palmitic 0.503 0.454
Stearic 0.627 0.49 3 0.424
Arachidic 0.60 0.056 0.139 0.494
Behenic 0.348 0.267* 0.120 0.589 0.598
lignoceric 0.486 0.206 0.057 0.473 0.208 0.640
Palmitoleic 0.617 0.858 0.288 0.393 -0.061 0.330 0.342
Oleic -0.212 -0.215* -0.316 0.052 0.212 0.096 0.011 -0.0113
Eicosenoic 0.323 0.008 0.097 0.313 0.187 0.358 0.633 0.138 0.18 4
Nervonic 0.024 -0.318 0.076 0.155 -0.023 0.057 0.397* -0.251 -0.047 0.407
Linoleic -0.524 -0.524 -0.566 -0.690 -0.396 -0.430 -0.306 -0.458 -0.478 -0.339 0.20
Linolenic -0.084 -0.102 -0.083 -0.0355*-0.243 -0.211 -0.169 -0.117 -0.368 -0.143 -0.137 0.352
*. Correlation is significant at the 0.01 level) 2 tailed). . Correlation is significant at the level (2 – tailed).
75000
50000
25000
0
10 20 30