Science - USA (2022-02-11)

Liuet al.,Science 375 , eabi5965 (2022) 11 February 2022 5 of 13

A

80

60

40

20

0

100

Saline

***

1.5

1.0

0.5

0

2.0

Saline

***

B

80

60

40

20

0

100

Cd55 Cd55

****

Cd55 Cd55

****

C

CD55

cDC2 Cd55

cDC1 Cd55

cDC2 Cd55

E

80

60

40

20

0

100

Donor

Recipient

Cd55 Cd55

Cd55 Cd55 Cd55

Cd55

***

n.s.

FG

0.04

0.03

0.02

0.01

0

0.05

cDC2 % in WBCs

Cd55 Cd55

**

H

80

60

40

20

0

100 n.s.

μMT:

Cd55

μMT:

Cd55

K

80

60

40

20

0

(^100) n.s.
TCRb
:Cd55
TCRb
:Cd55
I
RBCs
Cd55
Cd55
J
L
80
60
40
20
100
RBCs
Recipient
0
Cd55 Cd55
Cd55 Cd55 Cd55
Cd55
M
αCD55 αCD55
Cd55 + Cd55 RBCs
IgD DCIR2
cDC2 % in DCs
cDC2 % in splenocytes
0.03
0.02
0.01
0
0.04
cDC2 % in WBCs
Saline αCD55

• cDC2 % in DCs
  0
  1.5
  1.0
  0.5
  cDC2 % in splenocytes
  D
  cDC2 % in DCs cDC2 % in DCs cDC2 % in DCs
  cDC2 % in DCs
  cDC1 Cd55
  FO B Cd55
  cDC2 % in DCs
  n.s.

  ---

  
  


• 
  

  CD55
  SSC-H
  Cd55 + Cd55 RBCsCd55 + Cd55 RBCsCd55 + Cd55 RBCs
  32.4
  CD55 RBCs
  96.9 0.63
  N
  80
  60
  40
  20
  100
  0
  cDC2 % in DCs
  Mpl Mpl
  n.s.
  Rag1
  :Cd55
  Rag1
  :Cd55
  Cd55 + Cd55 RBCs Cd55 + Cd55 RBCs
  80
  60
  40
  20
  0
  100
  CD55
  Fig. 3. CD55 on RBCs is required for splenic cDC2 homeostasis.
  (A) Frequencies of cDC2s in (left) total DCs and (right) total splenocytes
  in WT mice treated with antibody to CD55 or saline. (B) Frequencies of
  cDC2s in blood of WT mice 8 hours after treatment with antibody to CD55 or
  saline. (C) Frequencies of cDC2s in (left) total DCs and (right) total
  splenocytes inCd55+/+orCd55−/−mice. (D) Frequencies of cDC2s in
  blood ofCd55−/−and control mice. (E) Representative histogram plots of
  surface CD55 on cDC2s, cDC1s, and B cells in mice with indicated
  genotype. (F) Frequencies of cDC2s in total DCs in chimeras as indicated.
  (G) Frequencies of cDC2s in total DCs in chimeras reconstituted with 90%
  mMT (IgM null) and 10%Cd55+/+orCd55−/−BM cells. (H) Frequencies of
  cDC2s in total DCs in chimeras reconstituted with 90%Tcrb−/−and 10%
  Cd55+/+orCd55−/−BM cells. (I) Frequencies of cDC2s in total DCs in
  chimeras reconstituted with 90%Rag1−/−and 10%Cd55+/+orCd55−/−
  BM cells. In (A) to (I), data are pooled from two independent experiments.
  (J) Representative histogram plots of surface CD55 on RBCs in mice with
  indicated genotype. (KtoM) RBC transfusions were performed from
  Cd55−/−orCd55+/+mice into the indicated recipient mice, and analysis was
  performed after 6 to 8 days. (K) Representative profile of purifiedCd55+/+
  RBCs orCd55−/−RBCs transfused intoCd55−/−orCd55+/+recipients. (L)
  Frequencies of cDC2s in total DCs in mice with purified RBCs transfused as
  indicated. Data are pooled from three independent experiments. (M)
  Distribution of DCIR2+cDC2s (red) and B cells (IgD, blue) in spleens as
  indicated. Scale bar, 200mm. Representative of multiple cross sections from
  at least three mice of each type. (N) Frequencies of cDC2s in total DCs in
  Mpl+/+orMpl−/−mice. Data are pooled from two independent experiments.
  Each symbol indicates one mouse, and lines denote means.
  P< 0.05; P< 0.01; P< 0.001; ****P< 0.0001.
  RESEARCH | RESEARCH ARTICLE