imaging analysis, J. Muppidi and X. Piao for helpful discussions,
and N. Carey for advice on statistical analysis.Funding:D.L. was
supported by a Cancer Research Institute Irvington Fellowship
and by funds from the UCSF Helen Diller Family Comprehensive
Cancer Center. E.L. was supported by NSF grant 1144247. A.M.
holds a Career Award for Medical Scientists from the Burroughs
Wellcome Fund and a Lloyd J. Old STAR award from the Cancer
Research Institute, is an investigator at the Chan Zuckerberg
Biohub, and received funds from Parker Institute for Cancer
Immunotherapy and the Innovative Genomics Institute. Z.Y. and
C.D.C.A. were supported by funds from The Sandler Asthma Basic
Research Center. J.G.C. is an investigator of the Howard Hughes
Medical Institute. This work was supported in part by NIH grant
AI040098 to J.G.C.Author contributions:D.L. and J.G.C.
conceived the project, designed the experiments, interpreted
the results, and wrote the manuscript. D.L. conducted the
experiments. L.D. analyzed the RNA-seq data. L.B.R. made initial
observations in ArhGEF1-deficient mice. E.L. performed RNA-seq of
ArhGEF1-deficient cDC2s. Y.X. performed molecular biology
experiments. J.A. genotyped mice. L.Q. and M.R.L. performed
spleen transplantation and IVC ligation from the UCSF
microsurgery core. F.L. and M.R.L. performed surgery to collect
blood from splenic artery and vein. Z.Y. and C.D.C.A. provided the
pQEF vector. Z.L., Y.X., and A.M. generated the CD97-deleted and
CD55-deleted mouse lines.Competing interests:J.G.C. is an
advisor for MiroBio and Be Biopharma and owns stock in ALX
Oncology. A.M. is a compensated cofounder, member of the boards
of directors, and a member of the scientific advisory boards of
Spotlight Therapeutics and Arsenal Biosciences. A.M. owns stock in
Arsenal Biosciences, Spotlight Therapeutics, Merck, and PACT
Pharma; A.M. is an investor in and informal advisor to Offline
Ventures.Data and materials availability:GSE77671 was used tocompare CD97-regulated genes in mice with splenic human
dendritic cells. Raw RNA-seq data have been deposited at GEO,
accession no. is GSE188835. All other data are available in the
main text or the supplementary materials.SUPPLEMENTARY MATERIALS
science.org/doi/10.1126/science.abi5965
Figs. S1 to S9
MDAR Reproducibility Checklist
Movies S1 to S821 March 2021; resubmitted 15 September 2021
Accepted 23 December 2021
10.1126/science.abi5965Liuet al.,Science 375 , eabi5965 (2022) 11 February 2022 13 of 13
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