T.W. implemented automatic filament picking and tools for data
conversion and statistical analysis. S.P. built atomic models for
actin, nebulin, and myosin motor domain. Z.W. performed rigid-body
fitting of myosin neck domain models. Z.W. prepared figures
with the assistance of M.Gr. Z.W., M.Gr., M.Ga., and S.R. wrote the
manuscript. All authors reviewed the results and commented on
the manuscript.Competing interests:The authors declare no
competing interests.Data and materials availability:Cryo-ET
structures are deposited in the Electron Microscopy Data Bank
under accession numbers EMD-13990 (skeletal A-band actin-
nebulin), EMD-13991 (skeletal actomyosin), EMD-13992 (skeletal
ELC-centered myosin), EMD-13993 (skeletal RLC-centered
myosin), EMD-13994 (skeletal I-band actin-nebulin), EMD-13995
(cardiac actin), EMD-13996 (cardiac actomyosin), and EMD-13997
(cardiac myosin neck domain). Representative tomograms are
deposited under accession numbers EMD-13998 (skeletal A-band),
EMD-13999 (skeletal I-band), and EMD-14000 (cardiac A-band).
The atomic models are deposited in the Protein Data Bank under
accession numbers 7QIM (skeletal actin-nebulin), 7QIN (skeletal
actomyosin), and 7QIO (skeletal myosin neck domain). Other
structures and EM density maps used in this study are available
under PDB 3I5G, 6KN8, and 6XE9 and EMD-0729.SUPPLEMENTARY MATERIALS
science.org/doi/10.1126/science.abn1934
Figs. S1 to S11
Table S1
MDAR Reproducibility Checklist
Movies S1 to S49 November 2021; accepted 12 January 2022
10.1126/science.abn1934Wanget al.,Science 375 , eabn1934 (2022) 18 February 2022 11 of 11
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