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ACKNOWLEDGMENTS
The authors wish to dedicate this work to the memory of
Bo A. G. Jönsson (1960–2016), whose dedication and knowledge was
instrumental to this project.Funding:This work received funding
from the European Union’s Horizon 2020 research and innovation
program under grant agreement no. 634880, EDC-MixRisk
(Å.B., J.R., C.-G.B., C.G., E.N., C.R., M.J., B.D., W.K., J.S., E.K., H.K.,
and G.T.); the European Research Council (ERC) (DISEASEAVATARS
616441 to G.T.); the EPIGEN Flagship Project of the Italian
National Research Council (CNR) (G.T.); the ERANET-Neurongrant from the Italian Ministry of Health (AUTSYN) (P.-L.G.); the
Umberto Veronesi Foundation (P.-L.G.); the Italian Ministry of Health
(Ricerca Corrente Grant to G.T.); ENDpoiNTs, European Union’s
Horizon 2020 research and innovation program (grant no. 825759
to G.T.); Science Without Borders Program (CAPES, Brazil) (R.B.B.);
the Swedish Research Council Formas (G.B. and J.R.); Centre
National de la Recherche Scientifique (CNRS) (J.-B.F. and B.D.); and
the Muséum National d’Histoire Naturelle (MNHN) (B.D. and J.-B.F.).
Author contributions:C.-G.B. is principal investigator for the
SELMA study and was responsible for the biostatistical modeling
together with C.G. N.C. carried out all the experiments with human
neurodevelopmental systems, with the help of M.T.R.; P.-L.G.
and C.C. designed the analytical strategy of transcriptomic data;
C.C., P.-L.G., and N.C. performed bioinformatic analysis; A.L.T.,
S.T., and N.C. set up the cortical organoids protocol; R.B.B. and
S.M.P. provided human fetal primary stem cells; N.C., M.T.R., and
F.C. performed the staining of organoids; E.T. and F.T. helped with
cortical organoid maintenance and library preparation; G.A.D.
helped with the graphical representation of the figures; L.B. carried
out all the experiments withD. rerio, including EDC exposure,
RT-qPCR, and the mobility assay; L.B. and J.S. analyzed the
D. reriodata; M.L. carried out all the experiments withX. laevis,
including EDC exposure, XETA, RT-qPCR, and the mobility assay;
B.D., J.-B.F., and M.L. analyzed theXenopusdata; M.L. and B.D. made
the Genomatix figure; N.E.C. and G.T. attended to the bioethical
issues of the project; C.L. contributed the chemical analysis and
preparation of the mix; Å.B., P.D., M.J., W.K., E.K., J.-B.F., H.K., M.Ö.,
P.R., C.R., E.E., E.N., G.B., M.Z., and O.S. contributed to the study
design, discussions, and critical reading of the manuscript; C.-G.B.,
C.G., N.C., C.C., B.D., P.-L.G., M.L., L.B., J.R., J.S., and G.T. wrote the
paper; and C.-G.B., B.D., J.-B.F., C.G., J.R., J.S., and G.T. conceived,
designed, and supervised the study.Competing interests:B.D. holds a
patent under the name“Transgenic clawed frog embryos and use
thereof as detectors of endocrine disruptors in the environment,”filed
in 2002 (number FR020669) and extended by a Patent Cooperation
Treaty filed in 2003 (patent number 8476484). All other authors
declare no competing interests.Data and materials availability:All
transcriptomic data have been made publicly available on the National
Center for Biotechnology Information’s Gene Expression Omnibus
(GEO) repository ( 111 ) and are accessible through GEO series accession
number GSE171566 (www.ncbi.nlm.nih.gov/geo/query/acc.cgi?
acc=GSE171566). All other data are available in the main text and
supplementary materials. All the bioinformatic analyses and the
code to reproduce the figures are available in a GitHub repository that is
publicly accessible to ensure complete reproducibility and to help the
reader to consult, understand, and reuse our data and analytical
pipelines [available at GitHub athttps://giuseppetestalab.github.io/
EDCMixRisk/and Zenodo ( 104 )]. Materials are available from the
corresponding authors upon requestSUPPLEMENTARY MATERIALS
science.org/doi/10.1126/science.abe8244
Materials and Methods
Figs. S1 to S9
Tables S1 to S9
References ( 112 – 133 )
MDAR Reproducibility Checklist15 November 2020; accepted 20 December 2021
10.1126/science.abe8244Caporaleet al.,Science 375 , eabe8244 (2022) 18 February 2022 15 of 15
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