330 Chapter 12
quality of these products can be signifi cantly
enhanced by the use of EMC without increas-
ing the quantity of cheese in a product.
EMC also can replace cheese in food for-
mulations; the levels used depend on the
amount of cheese being replaced and the
fl avor intensity of the EMC. For example, if
a current formulation has 50% cheese and a
50% substitution is desired with an EMC that
is 20 times more potent than the cheese
fl avor, only 1.25% of the EMC is required
to reach the substitution target without affect-
ing fl avor intensity. However, this reduces
the weight of the formulation, and other
ingredients such as starch and oil may haveIn some cases, cheese cultures are also
added. The resulting material has fi ve - to 15 -
fold strength of a regular cheese fl avor. The
exact reaction conditions and particulars are
closely held trade secrets. Figure 12.2 shows
the principles involved in EMC production.
After incubation to maximize the cheese
fl avor, the blend is heat treated to inactivate
the enzymes and cultures and spray dried.
EMC paste is also available.
A variety of fl avor profi les can be devel-
oped of varying compositions and product
forms. Typical applications include pro-
cessed cheese, cheese spreads, cheese dips,
cheese analogs, and cheese sauces. The fl avor
Figure 12.2. Principles of enzyme - modifi ed cheese production. Adapted from Wilkinson and Kilcawley (2003).
Comminuted
Cheese curd- CreamCone-bottom
TankCone-bottom
TankWater,
Emulsifying SaltsBlend,
Pasteurize,
CoolIncubate Mix-
Optimum Temp.,
pH, TimeProteases
and
PeptidasesHeat treat to
inactivate culture
and enzymesBlend,
Pasteurize,
CoolLipases/
EsterasesIncubate
Mix-
Optimum
Temp., pH,
TimeHeat treat to
inactivate culture
and enzymesEnzyme Modified
Cheese ConcentrateBlendCheese culture