Handbook of Meat Processing

Table 29.1. GMO specifi c PCR methods for GMO analysis. c PCR : conventional PCR ; RT i - PCR : real -
time PCR ; dc - PCR : double competitive PCR ; qc - PCR : quantitative competitive PCR. Modifi ed from
Hernandez et al. (2005)

GMO Target sequence Technique Reference

Maize event 176 cry IA(b), bla, bar , P - 35S/ bar,

ivr1

cPCR Ehlers et al., 1997

cry IA(b) nested PCR Studer et al., 1997

cry IA(b) and eukaryotic

genomic region

cPCR Hupfer et al., 1997

P - CDPK/ cry IA(b) nested PCR Hupfer et al., 1998

Soybean GTS40 - 3 - 2 P - 35S/CP4 - EPSPS , P - 35S/

CTP - EPSPS, le1

nested PCR Meyer & Jaccaud,

1997

P - 35S/CTP - EPSPS, CTP -

EPSPS/CP4 - EPSPS

nested PCR K ö ppel et al., 1997

P - 35S, T - nos cPCR van Duijn et al., 2002

CP4 - EPSPS/T - nos cPCR H ö rtner 1997

P - 35S/CTP - EPSPS dc - PCR Wurz and Willmund,

1997

P - 35S/CTP - EPSPS cPCR (validation) Anonymous, 1998

P - 35S/CTP - EPSPS and lectin RTi - PCR Wurz et al., 1999

Junction RTi - PCR Berdal and Holst -

Jensen, 2001

Junction RTi - PCR Terry and Harris,

2001

Junction RTi - PCR Taverniers et al.,

2001

EPSPS RTi - PCR Terry et al., 2002

EPSPS, le1 RTi - PCR

(validation)

Hird et al., 2003

EPSPS, CaMV, Nos ter, Even

specifi c, lectin

RTi - PCR Corbisier et al., 2005

Juntion, le1, soybean genome/

P35S

RTi - PCR Huang and Pan, 2005

Maize event 176 and

soybean GTS40 - 3 - 2

cry IA, EPSPS qc - PCR Studer, et al., 1998

cry IA(b), CP4 - EPSPS, zeine,

lectin

RTi - PCR Va ï tilingom et al.,

1999

P - 35S/peru - r1, cry IA, le1, ivr cPCR (validation) Jankiewicz et al.,

1999

P - 35S, T - nos, npt II, CTP,

cry IA(b), lectin, ivr

cPCR Vollenhofer et al.,

1999

Lectin, ivr , EPSPS, cry IA(b) cPCR Tengel et al., 2001

P - 35S and T - nos cPCR Mao et al., 2002

Lectin , lipid transfer protein

( ltp ) maize y P - 35S

RTi - PCR Alary et al., 2002

Maize MON810 P - 35S/ HSP 70 intron nested PCR Zimmermann et al.,

1998c

5 ′ junction RTi - PCR Holck et al., 2002

3 ′ junction RTi - PCR Hernandez et al.,

2003a

Maize MON863 zsSSIIb,p35S, TNOS, junction RTi - PCR Lee et al., 2006

Maize Bt11 cry IA(b) cPCR Ahl Goy 1998

cry IA(b) qc - PCR Zimmerman et al.,

2000

3 ′ junction and 5 ′ junction RTi - PCR R ø nning et al., 2003

Maize T25 Sintet. pat , P - 35S/ bla cPCR H ö chst et al., 1998

Maize GA21 Junction RTi - PCR Hernandez et al.,

2004a

Maize CBH - 351 cry 9C cPCR and RTi - PCR Matsuoka et al., 2001

Junction A, Junction B, cry 9c cPCR Windels et al., 2003

Maize NK - 603 Junction 5 ′ RTi - PCR Nielsen et al., 2004

Rapeseed Liberty Link PEPC cPCR Weiblinger et al.,

1999

(continued)

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