Table 29.1. GMO specifi c PCR methods for GMO analysis. c PCR : conventional PCR ; RT i - PCR : real -
time PCR ; dc - PCR : double competitive PCR ; qc - PCR : quantitative competitive PCR. Modifi ed from
Hernandez et al. (2005)
GMO Target sequence Technique Reference
Maize event 176 cry IA(b), bla, bar , P - 35S/ bar,
ivr1
cPCR Ehlers et al., 1997
cry IA(b) nested PCR Studer et al., 1997
cry IA(b) and eukaryotic
genomic region
cPCR Hupfer et al., 1997
P - CDPK/ cry IA(b) nested PCR Hupfer et al., 1998
Soybean GTS40 - 3 - 2 P - 35S/CP4 - EPSPS , P - 35S/
CTP - EPSPS, le1
nested PCR Meyer & Jaccaud,
1997
P - 35S/CTP - EPSPS, CTP -
EPSPS/CP4 - EPSPS
nested PCR K ö ppel et al., 1997
P - 35S, T - nos cPCR van Duijn et al., 2002
CP4 - EPSPS/T - nos cPCR H ö rtner 1997
P - 35S/CTP - EPSPS dc - PCR Wurz and Willmund,
1997
P - 35S/CTP - EPSPS cPCR (validation) Anonymous, 1998
P - 35S/CTP - EPSPS and lectin RTi - PCR Wurz et al., 1999
Junction RTi - PCR Berdal and Holst -
Jensen, 2001
Junction RTi - PCR Terry and Harris,
2001
Junction RTi - PCR Taverniers et al.,
2001
EPSPS RTi - PCR Terry et al., 2002
EPSPS, le1 RTi - PCR
(validation)
Hird et al., 2003
EPSPS, CaMV, Nos ter, Even
specifi c, lectin
RTi - PCR Corbisier et al., 2005
Juntion, le1, soybean genome/
P35S
RTi - PCR Huang and Pan, 2005
Maize event 176 and
soybean GTS40 - 3 - 2
cry IA, EPSPS qc - PCR Studer, et al., 1998
cry IA(b), CP4 - EPSPS, zeine,
lectin
RTi - PCR Va ï tilingom et al.,
1999
P - 35S/peru - r1, cry IA, le1, ivr cPCR (validation) Jankiewicz et al.,
1999
P - 35S, T - nos, npt II, CTP,
cry IA(b), lectin, ivr
cPCR Vollenhofer et al.,
1999
Lectin, ivr , EPSPS, cry IA(b) cPCR Tengel et al., 2001
P - 35S and T - nos cPCR Mao et al., 2002
Lectin , lipid transfer protein
( ltp ) maize y P - 35S
RTi - PCR Alary et al., 2002
Maize MON810 P - 35S/ HSP 70 intron nested PCR Zimmermann et al.,
1998c
5 ′ junction RTi - PCR Holck et al., 2002
3 ′ junction RTi - PCR Hernandez et al.,
2003a
Maize MON863 zsSSIIb,p35S, TNOS, junction RTi - PCR Lee et al., 2006
Maize Bt11 cry IA(b) cPCR Ahl Goy 1998
cry IA(b) qc - PCR Zimmerman et al.,
2000
3 ′ junction and 5 ′ junction RTi - PCR R ø nning et al., 2003
Maize T25 Sintet. pat , P - 35S/ bla cPCR H ö chst et al., 1998
Maize GA21 Junction RTi - PCR Hernandez et al.,
2004a
Maize CBH - 351 cry 9C cPCR and RTi - PCR Matsuoka et al., 2001
Junction A, Junction B, cry 9c cPCR Windels et al., 2003
Maize NK - 603 Junction 5 ′ RTi - PCR Nielsen et al., 2004
Rapeseed Liberty Link PEPC cPCR Weiblinger et al.,
1999
(continued)
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