103

DNA and

DNA

Evidence*

Brion C. smith

david sweet o.C.

Contents
7.1 Introduction 104
7.2 Molecular Biology and Inheritance 1 04
7.3 Laboratory Processing 1 06
7.3.1 Extraction (Isolation of DNA) 1 06
7.3.2 Amplification (Quantitation and PCR) 1 08
7.3.3 Analysis (Electrophoresis, Detection, and Interpretation) 111
7.3.4 DNA Laboratory Quality Assurance 1 13
7.4 DNA and the Management of Mass Fatality Incidents 1 14
7.4.1 Planning 115
7.4.2 Establishing the Scope 1 16
7.4.3 Communicating with the Laboratory 1 16
7.4.4 Evidence Collection Teams 1 17
7.4.5 Transportation and Storage 1 18
7.4.6 Data Management 1 19
7.5 Teeth as Sources for Forensic DNA 1 19
7.6 Saliva and Oral Mucosa as Sources for Forensic DNA 120
7.6.1 Salivary DNA in Bitemark Evidence 121
7.6.2 Oral Mucosal DNA and Buccal Swabs 1 23
7.7 DNA Databases 1 25
7.7.1 CODIS 126
7.7.1.1 Convicted Offender Index 126
7.7.1.2 Forensic Index 126
Glossary 127
Acknowledgments 131
References 131
Suggested Reading 135

*^ the opinions and assertions contained herein are solely those of the authors and are

not to be construed as official or as views of the u.s. department of defense, the u.s.

department of the army, or the armed Forces institute of pathology.

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