supply and the bag containing the object. This allows the “dry” nitrogen
from the cylinder to be humidified to the object’s optimal RH before the
gas flows into the bag. The humidification system functions by dividing
the gas flow from the nitrogen cylinder into two valve-controlled lines.
One stream of nitrogen is bubbled through water contained in a stout
polypropylene bottle. The second stream flows directly to a second (dry)
bottle, which is also connected to the water-filled bottle. The mixing of
the dry and humidified gases is controlled by valves, which regulate the
flow rate into each bottle. To monitor the RH of the resulting combined
gas stream, a third bottle is used that contains an RH sensor and that also
acts as a final mixing chamber before the humidified gas passes into the
plastic bag containing the object (Fig. 7).
An important aspect ofthe design ofthe nitrogen-supply-and-
humidification system is the use of leakprooffittings that minimize the
influx ofoxygen into the system. All fittings from the nitrogen cylinder to
the entrance ofthe bag use^1 / 4 -in. (approx. 6 mm) brass O-ring-sealed
Swagelok fittings. These fittings connect the polypropylene tubing, which
is used to pipe the nitrogen gas from the gas cylinder, through the humidi-
fication system, and into the bag. Swagelok O-ring-sealed fittings are
inserted into holes that are precisely drilled in the lids of the humidifica-
74 Hanlon and Daniel
Aclar bag
Humidification system
Nitrogen
supply
Figure 6
Schematic of the dynamic system for creating
a low-oxygen environment.