tion bottles and also connect the polypropylene piping joining the three
bottles. ASwagelok fitting is also inserted into a precisely cut hole in the
Aclar bag to allow the pipe to form a leakproof connection into the bag.
AT fitting with an on/offvalve is attached between the third bottle of
the humidification system (which houses the RH sensor) and the bag. This
allows the release ofthe nitrogen gas into the room atmosphere during
the balancing of the humidification system, to produce the desired level
of humidification ofthe nitrogen flow.
During the initial flushing at a high flow rate, the RH inside the
bag should be constantly monitored. An opening of15–30 cm is left
unsealed and open on the corner of the bag opposite the nitrogen inlet to
allow efficient mixing and flushing of the interior atmosphere without
pressurizing the bag (Fig. 8). This opening is heat-sealed after the desired
stable oxygen concentration is achieved.M A T I I 75Valves for controlling amounts
of “wet” and “dry” nitrogenNitrogen bubbles
through water“Dry” nitrogenRH monitoredNitrogen
supply
from cylinderHumidified nitrogen
piped to bag
enclosing objectFigure 7
Humidification system for the nitrogen gas.
High flow rate
of nitrogen
into bag15 – 30 cm of
seam left open
during purgeFigure 8
System for purging the encapsulating bag
with nitrogen gas to reduce the oxygen con-
centration to 0.1%.