Food Biochemistry and Food Processing (2 edition)

(Wang) #1

BLBS102-c03 BLBS102-Simpson March 21, 2012 11:56 Trim: 276mm X 219mm Printer Name: Yet to Come


44 Part 1: Principles/Food Analysis

Table 3.2.Enzymatic Methods for Food Component Analysis

Food Component Enzymes Involved Commercial Kit Reference

Amino Acids
Amino acids Amino acid decarboxylase, amino
acid oxidase Wiseman 1981, Mello and
Kubota 2002,
Prodromidis and
Karayannis 2002, Cock
et al. 2009

Tryptophan Tryptophanase
Aspartic acid Aspartate aminotransferase
Glutamic acid Glutamate dehydrogenase, glutamate
oxidase, diaphorase

Megazyme International

Lysine Lysine oxidase

Carbohydrates
Sucrose Invertase, hexokinase,
glucose-6-phosphate
dehydrogenase

Megazyme International
Sigma Aldrich, Inc.

Fructose Hexokinase, phosphoglucose
isomerase, glucose-6-phosphate
dehydrogenase, fructose
dehydrogenase

Sigma Aldrich, Inc.
Megazyme
International

Glucose Glucose oxidase, peroxidase,
hexokinase, glucose-6-phosphate
dehydrogenase

Cayman Chemical Co.
Sigma Aldrich, Inc.
Megazyme
International

Wiseman 1981, Mello and
Kubota 2002,
Prodromidis and
Karayannis 2002, Cock
et al. 2009
Lactose β-Galactosidase Megazyme International
Lactulose Fructose dehydrogenase,
β-galactosidase
Raffinose α-Galactosidase, galactose
mutarotase,β-galactose
dehydrogenase

Megazyme International

Starch α-Amylase, amyloglucosidase,
glucose oxidase, peroxidase,
amyloglucosidase, hexokinase,
glucose-6-phosphate
dehydrogenase

Megazyme International
Sigma Aldrich
Company

Dietary fiber, dextran,
and hemicellulose

α-Amylase, protease,
amyloglucosidase, dextranase,
hemicellulase

Sigma Aldrich, Inc.

Pectin Pectate lyase Megazyme International

Lipids
Triglycerides Lipase, glycerol kinase, glycerol
phosphate oxidase, peroxidase,
lipoprotein lipase, glycerol kinase,
glycerol phosphate oxidase

Cayman Chemical Co.
Sigma Aldrich, Inc.

Cholesterol Cholesterol esterase, cholesterol
oxidase, peroxidase

Cayman Chemical Co.

Glycerol Glycerol kinase, glycerol phosphate
oxidase, peroxidase, glycerol
dehydrogenase,
glycerol-1-phosphate
dehydrogenase, pyruvate kinase,
lactate dehydrogenase

Megazyme International
Cayman Chemical Co.
Sigma Chemical Co.

Wiseman 1981, Mello and
Kubota 2002,
Prodromidis and
Karayannis 2002, Cock
et al. 2009

Phosphatidyl choline
(lecithin)

Phospholipase D, choline oxidase,
peroxidase

Cayman Chemical Co.

Fatty acids Lipases
Free download pdf