BLBS102-c32 BLBS102-Simpson March 21, 2012 14:2 Trim: 276mm X 219mm Printer Name: Yet to Come
Table 32.2.
Summary of Transgenic Approaches to Manipulate Starch Structure and Yield in Potato Tubers
Transgenic
Starch
Grain/Tuber
Target
Approach
Content/ Yield
Starch Structure
Morphology
Application
Reference
Plastidial ATP/ADP
translocator
Antisense inhibition Starch content
decreased to 20%
Less amylose
Grains size
decreased to 50%,strange tubershape
—
Tjaden et al. 1998
Plastidial ATP/ADP
translocator
Overexpression of
AATP
Starch content
increased by up to30%
More amylose
Grains with angular
shape
High starch with
more amylose
Tjaden et al. 1998,
Geigenbergeret al. 2001
Plastidial adenylate
kinase
Antisense inhibition Starch content and
yield increased byup to 60%
Not determined
Not determined
High starch
Regierer et al. 2003
ADPGlc pyro-
phosphorylase
Antisense inhibition
of AGPB
Starch content
decreased down to10% of WT-level
Amylose content
decreased by 40%
Smaller grains,
smaller tubers,increased tubernumber
—M
̈uller-R
̈ober et al.
1992, Lloyd et al.1999b
ADPGlc pyro-
phosphorylase
Overexpression of
glgC
Starch content
increased by up to30%
Not determined
Not determined
High starch
Stark et al. 1991
Granule bound
starch synthase
Antisense inhibition
of GBSSI
No effect
Starch is free of
amylose
No effect
Amylose free starch Kuipers et al. 1994
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