inorganic chemistry

TABLE V

PHOTOSENSITIZER

P

ARAMETERS AND

L

IGHT

D

OSE

(h

n^90

)U

NDER

B

ROAD

-B

AND

IRRADIATION

R

EQUIRED TO

K

ILL

90%

OF

M

ELANOMA

(S91

OF

SKMEL-188)

OR

C

OLON

C

ARCINOMA

(HT29) C

ELLS

IN

V

ITRO

Sensitizer/cell line

l

(nm)

F

D

e

(M



1

cm



1 ) LD

50Dark

(m

M)

a

LD

90PDT

(m

M)

hn

90

(J/cm

2 ) LD

90

hn

90

(m

M J/cm

2 )

Photofrin/S91

b

630

0.36

c

1170

>

25

17

0.175

3.0

H

TClPPOH/S91 2

d

633

e

0.74

e

504

e

>

200

20

4.0

80.0

H

TClPBOH/S91 2

f

748

e

0.43

e

23,900

e

>

200

5

0.26

1.3

H

TClPPOH/SKMEL 2

d

633

e

0.74

e

504

e

>

200

20

5.4

108

H

TClPCOH/SKMEL 2

g

650

e

0.86

e

6955

e

>

200

20

0.46

9.2

H

TClPBOH/SKMEL 2

g

742

h

0.42

h

61,000

h

5

0.32

1.6

Tookad/HT29

i

763



1



100,000

>

5

0.5

12

6.0

aThe concentrations employed in these studies were not sufficiently high to induce 50% cell death in the dark, but the lower limits presented arehigher than the onset of dark toxicity (10

• 20% cell death).

bFrom Refs. (

96

) and (

102

), the concentrations employed in PDT, 10

mg/ml, were converted to molar units using the nominal molar weight of the

mixture (600 g/mol)

(^104

) given that the molar weight of Photofrin is ill-defined.

cFor a 10-

mM PBS solution of hematoporphyrin derivative

(^97

).

dFrom Ref. (

54

).

eIn aqueous solutions.fFrom Ref. (

105

).

gFrom

Ref. (

100

).

hIn ethanol from

Ref. (

90

).

iFrom Refs. (

106

) and (

79

), photophysical data in acetone.