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Table 8.4.

Comparison of the Main Expression Systems

Mammalian

Transgenic

Transgenic

Bacteria

Yeast

Fungi

Insect Cells

Cells

Plants

Animals

Developing time

Short

Short

Short

Intermediate

Intermediate

Intermediate

Long

Costs for downstream















processing

Levels of expression

High

Intermediate

Intermediate

Intermediate

Low

Low

Low

Recombinant protein





stability

Production volume

Limited

Limited

Limited

Limited

Limited

Unlimited

Unlimited

Posttranslational No

Yes

Yes

Yes

Yes

Yes

Yes

modifications (disulphide

bond formation,glycosylation, etc.)

“Human-type” No

No

No

No

Yes

No

Yes

glycosylation

Folding capabilities

—













Contamination level



———



—



(pathogens, EPL, etc.)