18 Proteomics in Fish Processing 411Table 18.2.Free Amino Acids and NH 3 Levels in Muscle of Trout Fed a Fishmeal–Containing
Diet (FM) or a Plant Protein–Containing Diet (PP) at 0 and 9 Days after Death0 d FM 9 d FM 0 d PP 9 d PP
FAA NH 3 (n 3) (n 3) (n 3) (n 3)
(mg/100 g fresh muscle) (Mean SD) (Mean SD) (Mean SD) (Mean SD)D,O-phosphoserine 0.520.19 0.470.22 0.490.05 1.100.25
O-phosphoethanolamine NDa 0.220.44 ND ND
Taurine 91.0548.32 96.6826.21 66.0122.48 95.0027.40
Aspartic acid 0.520.30 0.320.18 0.210.22 0.740.30
Threonine 6.361.24 6.811.37 6.942.65 11.941.91
Serine 4.571.66 6.412.03 2.921.39 4.000.40
Asparagine 7.332.43 ND 5.030.83 ND
Glutamic acid 8.612.87 13.032.61 6.411.33 13.925.51
Glutamine 22.742.51 3.243.78 14.793.27 ND
Proline 2.030.90 5.122.27 17.102.14 14.5910.11
Glycine 61.187.43 93.9921.81 76.5042.51 81.8530.06
Alanine 11.486.80 16.882.18 13.331.73 24.335.14
Citrulline 0.220.30 0.220.26 1.760.94 0.190.24
2-amino-n-butyric acid 0.210.08 0.190.06 0.410.12 0.380.06
Valine 3.550.85 4.351.02 3.840.62 5.641.08
Cystine ND 0.030.06 ND ND
Methionine 1.990.63 2.600.67 1.820.21 2.160.26
Cystathionine ND 0.170.06 0.130.05 0.170.02
Leucine 1.790.54 2.350.61 1.670.37 2.880.65
Isoleucine 2.880.55 3.720.77 2.810.43 4.490.68
Phenylalanine 1.060.45 1.890.72 1.260.15 2.020.28
Tyrosine 1.650.74 2.110.60 1.460.75 2.030.23
-alanine 3.031.72 4.090.88 5.642.29 8.633.78
D-2-amino-isobutyric acid 0.090.16 ND 0.060.11 0.120.21
D-homocystine 0.020.03 0.030.06 0.150.15 0.090.08
D-4-amino-butyric acid 1.650.12 1.360.38 4.103.92 5.221.30
Tryptophane ND ND ND ND
Ethanolamine ND ND ND ND
Allo-hyd ND ND ND ND
D-hydroxylysine ND ND ND ND
NH 3 43.481.34 47.685.12 47.165.17 52.331.37
Ornithine 2.741.29 1.550.66 1.850.53 5.085.00
Lysine 31.5424.53 18.176.74 15.435.37 22.463.09
Histidine 32.0315.42 39.293.91 147.4915.71 127.4021.45
3-methyhistidyne 0.060.02 0.060.05 0.140.25 0.080.14
1-methyhistidyne 0.570.35 3.881.25 0.390.20 5.582.34
AnserineL-carnosine 466.4650.44 398.4623.85 515.03132.25 451.9321.90
Arginine 9.504.64 5.602.68 4.771.20 7.861.35
873.62 834.55 1022.85 1013.70
aND identity not determined.