Food Biochemistry and Food Processing

412

Table 18.3.

Protein Spots Affected by Dietary Plant Protein Substitution in Rainbow

Trout as Judged by 2DE and Their Identities as

Determined by Trypsin Digest Mass FingerprintingSpot

Normalized

Normalized

Reference

MW

Volume

Volume

Fold

No.

pH

(kDa)

Diet FM

a

Diet PP100

a

Difference

P

Downregulated 128

6.3

66

303



57

60



19

Vacuolar ATPase

5

0.026

-subunit

291

6.4

42

521



37

273



30

-ureidopropionase

2

0.004

356

6.3

38

161



37

44



19

Transaldolase

4

0.031

747

5.6

43

101



19

19



11

-actin

2

0.040

760

6.3

39

41



62

1 

5N

D

b

2

0.040

766

4.8

27

12



16



1

ND

2

0.004

Upregulated 80

4.4

82

9



4

47



8

“Unknown protein”

5

0.007

87

5.7

75

58



14

262



21

Transferrin

5



0.001

138

5.5

67

99



16

267



39

Hemopexin-like

3

0.009

144

5.4

63

26



6

265



66

L-Plastin

10

0.018

190

5.9

54

6



2

50



9

Malic enzyme

9

0.018

199

5.9

53

60



16

156



13

Thyroid hormone

3

0.020

receptor

275

6.1

45

1



0.6

11



0.6

NSH

b

9



0.001

387

5.6

35

97



3

251



49

Electron transferring

3

0.035

flavoprotein

389

5.8

35

192



45

414



54

Electron transferring

2

0.027

flavoprotein

399

6.8

33

59



12

130



10

Aldolase B

2

0.028

457

4.7

29

26



7

57



5

14-3-3 B2 protein

2

0.021

461

4.7

27

75



9

190



12

Proteasome alpha 2

3

0.004

517

4.4

22

15



6

135



29

Cytochrome

c

oxidase

9

0.013

539

4.9

19

7



3

18



3

ND

3

0.033

551

4.1

17

40



11

143



28

ND

4

0.018

563

5.2

15

814



198

3762



984

Fatty acid binding

5

0.039

protein