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ACKNOWLEDGMENTS
We thank T. Beeckman, Y. Eshed, Z. Lippman, and S. Savaldi-
Goldstein for comments and discussions; M. De Martino for setting
up the CRISPR system; I. Pri-Tal for tissue culture work; O. Roth
for assistance in etiolated seedlings assay; and M. Chemla for
assistance in library preparation.Funding:I.E. is supported by a
Howard Hughes Medical Institute International Research Scholar
grant (grant no. 55008730) and the Israeli Science Foundation
(grant no. ISF966/17). A.H. is supported by National Science
Foundation grant Plant Genome Research Program grant IOS-
1546837 to Z.B.L.Author contributions:M.O., N.G.-Y., and I.E.
conceived and designed the study and performed the experiments.
C.Y. performed the shoot-borne root quantification and lateral root
RNA-Seq assay. A.H. performed in situ hybridizations. E.S.
performed potato transformation. I.E., N.G.-Y., and M.O. wrote the
manuscript.Competing interests:The authors declare no
competing interests.Data and materials availability:Raw and
processed data are available at the Gene Expression Omnibus
(GEO; single-cell data: GSE159055; lateral root transcriptome data:
GSE159050). All other data are available in the main paper or the
supplementary materials.SUPPLEMENTARY MATERIALS
science.org/doi/10.1126/science.abf4368
Materials and Methods
Figs. S1 to S14
References ( 47 – 68 )
Tables S1 to S6
MDAR Reproducibility Checklist2 November 2020; resubmitted 17 October 2021
Accepted 13 January 2022
10.1126/science.abf4368Omaryet al.,Science 375 , eabf4368 (2022) 4 March 2022 7of7
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