Liet al.,Science 375 , eabk2432 (2022) 4 March 2022 3 of 12
Fig. 2. Cell-type annotation for dissected tissues.(A) Illustration of 15 indi-
vidual tissues: Twelve are sequenced separately from males and females and
three are sex-specific. Fat body, oenocyte, and tracheal nuclei were labeled
using a tissue-specific GAL4 driving UASÐnuclear-GFP. (B) tSNE plot with
annotations for the body wall from the Stringent 10x dataset. 1, epidermal cells
of the abdominal posterior compartment; 2, epidermal cells specialized in
antimicrobial response. (C) Uniform manifold approximation and projection
(UMAP) plot with annotations for the testis from the Relaxed 10x dataset.
(D) tSNE plots of the other 13 tissues from the Stringent 10x dataset. Detailed
annotations are in figs. S6 to S18. (E) Number of unique annotations for each
tissue. Fractions of annotated cells over all analyzed cells from the Relaxed
dataset are indicated in red.RESEARCH | RESEARCH ARTICLE