Liet al.,Science 375 , eabk2432 (2022) 4 March 2022 5 of 12
Fig. 3. Whole-head and whole-body sequencing leads to full coverage of
the entire fly.(A) tSNE of the whole-head sample with 81 annotated clusters.
See fig. S22 for full cell types. Many cells in the middle (gray) are unannotated,
most of which are central brain neurons. (B) tSNE of the whole-body sample
with 33 annotated clusters, many of which can be further divided into subclusters.
Cells in gray are unannotated. See fig. S23 for full cell types. (C) tSNE of the
entire dataset colored by standardized tissue enrichment, leading to the
identification of head- and body-specific clusters, is shown on the left. Stacked bar
plot showing tissue composition (head, body, or dissected tissues) for different
clusters at Leiden resolution 50 are shown on the right. (D) Examples of head-
and body-specific clusters. (E) Integration of a brain scRNA-seq dataset with
the head snRNA-seq for label transfer. Outlined are example clusters revealed
by the head snRNA-seq dataset but not by the brain scRNA-seq datasets,
including epithelial cells (EPI), photoreceptors (PRs), olfactory receptor neurons
(ORNs), and muscle cells (MUS). (F) Subclustering analysis reveals types of
photoreceptors, including inner and outer photoreceptors, with the inner
photoreceptors further splitting into R7 and R8 types, and mushroom body
Kenyon cells comprising three distinct types:a/b,a′/b′, andg.RESEARCH | RESEARCH ARTICLE