8-62
f. Examine each test tube for agglutination.
(1) Write on the work sheet “+” for agglutination.
NOTE: Grade the degree of agglutination: +/=, 1+, 2+, 3+, or 4+.
(2) Write on the work sheet “=” for no agglutination.
NOTE: Current standards do not require the use of optical aids before a crossmatch may be
considered compatible, but their use may enhance sensitivity and consistency.
g. Add check cells to all negative tubes to confirm the reactivity of the antiglobulin reagent.
NOTE: If the negative tubes do not show agglutination after adding check cells, repeat the bovine
albumin phase (steps 8 through 10g).
- Obtain correct results for the specimen given.