Instant Notes: Analytical Chemistry

(Tina Meador) #1
Conductance monitorsare used in ion chromatography, a mode of HPLC
useful for separating low levels of inorganic and organic anions and cations
by ion-exchange (Topic D7).

These aspects of HPLC closely parallel those described for GC (Topic D4).
Additional instrument parameters to be set and monitored include:

● solvent composition, flow rate and pressure limit;
● gradient elution programs;
● wavelength(s) and wavelength-switching for UV-visible absorbance detec-
tors;
● wavelength range, sampling frequency and mode of display for a DAD.

Instrument
control and data
processing


D6 – HPLC: principles and instrumentation 165

Free download pdf