172 Section D – Separation techniques
1(^32)
6
4
5
Methanol (Meth) 7.5%
Tetrahydrofuran (THF) 42.5%
Water 50%
Meth 4.5%
THF 25.5%
Water 70%
1
2
3
(^65)
4
Meth 10%
THF 25%
Water 65%
(^12)
3
6
5
4
Meth 31.8%
THF 21.2%
Water 47%
1
2
3
(^64)
5
Meth 0%
THF 35%
Water 65%
(^132)
6
(^54)
Fig. 5. Optimizing an HPLC separation using ternary mobile phases. Solutes: 1. benzyl alcohol; 2. phenol; 3. 3-phenyl-
propanol; 4. 2,4-dimethylphenol; 5. benzene; 6. diethyl o-phthalate.