Steps in Automated Analysis Clinical Chemistry Review 79
STEP COMMENTS
Sample ID Usually by bar code reader
Test selection Usually communicated by LIS
Sampling Usually closed-tube sampling from primary collection tubes. Some analyzers have short sample & clot detection
Reagent delivery Usually by syringes, pumps, or pressurized reagent bottles. Vitros uses dry slides. Some offer reagent inventory
Chemical reaction Mixing & incubation
Measurements Visible & UV spectrophotometry, ion selective electrodes, fluorescence polarization, chemiluminescence,
bioluminescence. Most offer automatic dilution & retesting when linearity is exceeded
Data handling Concentration derived from calibration curve stored in analyzer
Reporting Usually reported to LIS through interface
Troubleshooting Can be done remotely by modem on many analyzers