Quick Review Cards for Medical Laboratory Science

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Special Bacteriologic Media Clinical Microbiology Review 153


MEDIUM USE OTHER

Cystine-tellurite blood agar


Loeffler medium


Tindale agar


Bismuth sulfite agar


Cefsulodin-irgasan-novobiocin
(CIN) agar


Alkaline peptone water (APW)


C. diphtheriaeproduces black colonies.

Promotes development of metachromatic granules.

Corynebacteriumspp produce gray to black colonies due to
reduction of tellurite. C. diphtheriaecolonies are surrounded
by a brown halo.

Bismuth sulfite & brilliant green inhibit most others. S. typhi
colonies are black, surrounded by metallic sheen. Others are
light green. (Some salmonellae may be inhibited.)

Crystal violet inhibits most GN. Novobiocin inhibits GPC.
Cefsulodin inhibits most GP & GN. Y. enterocoliticaferments
mannitol, appears as red “bull’s-eye” colonies surrounded by
colorless halo.

Alkaline pH suppresses commensals. Subcultured to TCBS.

Differential medium for isolation of
Corynebacterium diphtheriae

Enrichment medium for C. diphtheriae

Selective differential medium for C.
diphtheriae

Selective for Salmonella

Selective medium for Yersinia entero-
colitica, Aeromonas,& Plesiomonas
shigelloides

Enrichment medium for recovery of
Vibriofrom stool

continued...
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