Quick Review Cards for Medical Laboratory Science

Special Bacteriologic Media Clinical Microbiology Review 153

MEDIUM USE OTHER

Cystine-tellurite blood agar

Loeffler medium

Tindale agar

Bismuth sulfite agar

Cefsulodin-irgasan-novobiocin
(CIN) agar

Alkaline peptone water (APW)

C. diphtheriaeproduces black colonies.

Promotes development of metachromatic granules.

Corynebacteriumspp produce gray to black colonies due to

reduction of tellurite. C. diphtheriaecolonies are surrounded

by a brown halo.

Bismuth sulfite & brilliant green inhibit most others. S. typhi

colonies are black, surrounded by metallic sheen. Others are

light green. (Some salmonellae may be inhibited.)

Crystal violet inhibits most GN. Novobiocin inhibits GPC.

Cefsulodin inhibits most GP & GN. Y. enterocoliticaferments

mannitol, appears as red “bull’s-eye” colonies surrounded by

colorless halo.

Alkaline pH suppresses commensals. Subcultured to TCBS.

Differential medium for isolation of

Corynebacterium diphtheriae

Enrichment medium for C. diphtheriae

Selective differential medium for C.

diphtheriae

Selective for Salmonella

Selective medium for Yersinia entero-

colitica, Aeromonas,& Plesiomonas

shigelloides

Enrichment medium for recovery of

Vibriofrom stool

continued...