Special Bacteriologic Media continued Clinical Microbiology Review 154
MEDIUM USE OTHER
Thiosulfate citrate bile salts
sucrose (TCBS) agar
Bordet-Gengou agar
Regan-Lowe agar
Buffered charcoal-yeast
extract (BCYE) agar
Human blood bilayer Tween
(HBT) agar
High pH inhibits most bacteria. V. choleraeferments sucrose,
produces yellow colonies. V. parahaemolyticus& V. vulnificus
don’t ferment sucrose; usually produce blue-green colonies.
Potato-glycerol-based medium enriched with blood. Contam-
inants inhibited by methicillin. “Cough plate.” Bordetella
colonies resemble mercury droplets.
Charcoal agar supplemented with horse blood, cephalexin, &
amphotericin B.
Yeast extract & L-cysteine enhance growth of Legionella.
Charcoal absorbs toxic compounds.
Incubate in ↑CO 2 for 48 hr. Colonies are beta hemolytic.
Selective for Vibrio
Selective enrichment medium for
isolation of Bordetella pertussis
Selective for B. pertussis
Enrichment medium for isolation of
Legionella
Selective & differential for G. vaginalis
