Hemolytic Reactions on Sheep Blood Agar Clinical Microbiology Review 157
HEMOLYSIS DESCRIPTION EXPLANATION EXAMPLES
Alpha
Beta
Gamma (nonhemolytic)
Streptococcus pneumoniae, viridans
streptococci, some enterococci
Group A strep, group B strep, Listeria
monocytogenes
Some enterococci
Green zone around colony.
May be narrow or wide.
Clear zone around colony.
May be narrow or wide.
No zone of hemolysis.
Partial lysis of RBCs
Complete lysis of RBCs
No lysis of RBCs