Characteristics of Enterobacteriaceae Clinical Microbiology Review 176
Gram stain Non–spore-forming gram-negative rods
Growth characteristics Facultative anaerobes
Colonies on sheep blood agar Most are large, dull, gray, nonhemolytic
Colonies on MacConkey Lactose fermenters pink
Non–lactose fermenters colorless
Biochemicals Ferment glucose
Oxidase neg
Most reduce nitrates to nitrites
Most are catalase pos