Media for Culture of Anaerobes Clinical Microbiology Review 199
MEDIUM USE
Anaerobic blood agar (CDC)
Bacteroidesbile–esculin (BBE) agar
Brucellablood agar
Colistin–nalidixic acid (CNA) blood agar
Cycloserine cefoxitin fructose egg yolk
(CCFA) agar
Egg-yolk agar (EYA)
Kanamycin-vancomycin laked blood
(KVLB) agar
Phenylethyl alcohol (PEA) agar
Thioglycolate (THIO) broth
Nonselective enrichment medium that grows obligate & facultative anaerobes. Contains
yeast extract, L-cysteine, hemin, & vitamin K.Selective differential medium for Bacteroides fragilis. Bile salts & gentamicin act as inhibitors.
B. fragiliscolonies black with dark halos due to esculin hydrolysis.Enriched medium that grows obligate & facultative anaerobes.Selective medium that grows obligate anaerobes & GP facultative anaerobes.Selective & differential for Clostridium difficile. Colonies are yellow due to fermentation of
fructose. Chartreuse fluorescence.For determination of lecithinase & lipase production by clostridia & fusobacteria.Also known as laked blood kanamycin-vancomycin (LKV) agar. Most commonly used selective
medium for isolation of anaerobic GNRs, especially Bacteroides & Prevotella.Selective medium that inhibits enteric GNRs & grows obligate anaerobes & GP facultative
anaerobes.All-purpose medium that supports growth of most aerobes & anaerobes. Can be used as
backup broth to detect organisms present in small numbers or anaerobes. Thioglycolate acts
as reducing agent. Aerobes grow at top, strict anaerobes at bottom, facultative anaerobes
throughout. Store at RT. Boil & cool before use.