Quick Review Cards for Medical Laboratory Science

Cell Cultures Clinical Microbiology Review 285

CELL LINE DERIVATION EXAMPLES FOR ISOLATION OF

Primary Cells from mammalian tissue Primary monkey kidney (PMK) cells Most viruses

Finite (diploid) Cultures of connective tissue cells Human neonatal lung (HNL) CMV (only type of cell

culture that can be used)

Continuous (immortal, Malignant or transformed cells Hep2, A549, Vero HSV, RSV, enteroviruses,

heteroploid) adenoviruses

No single cell type grows all viruses. Several types should be inoculated.