Quick Review Cards for Medical Laboratory Science

Coagulation Instrumentation Hematology Review 362

ENDPOINT DETECTION PRINCIPLE

Mechanical Change in electrical conductivity between 2 probes or change in movement of steel ball when clot forms

Photo-optical ↓in light transmittance as fibrin forms

Chromogenic ↑in light absorbance at 405 nm as para-nitroaniline (pNA) is cleaved from synthetic substrate by coag

enzyme

Immunologic ↑in light absorbance as latex particles coated with specific antibody are agglutinated by antigen

Some analyzers have multiple detection methods.