Isolation & Identification of Lymphocytes Immunology Review 380
Isolation Density gradient centrifugation with Ficoll-Hypaque. Layers from top to bottom: plasma, mononuclear cells,
Ficoll-Hypaque, RBCs, & granulocytes.
Identification Flow cytometry. Fluorescent-labeled monoclonal abs against specific surface ags. Each ab has different fluorescent
tag. Light scattering measured as cells flow through laser beam. Common T-cell ags tested: CD2, CD3, CD4, CD7,
CD8; B-cell ags: CD19, CD20, CD22, surface Ig.
