Comparison of Labeled
Immunoassayscontinued
Immunology Review 396
Disadvantages
Use
CHEMILUMINESCENT
IMMUNOASSAY (CIA)
Quenching of light emis-
sion by some biological
materials.
Common.
FIA
Autofluorescence from
organic substances in
serum. Nonspecific bind-
ing to substances in
serum. Expensive, dedi-
cated instrumentation.
Common.
RIA
Radiation hazard.
Short shelf life of
reagents. Disposal
problem. Licens-
ing & federal
regulations.
Not common.
EIA
Natural inhibitors in some
specimens. Nonspecific
protein binding.
Common.
