Collection of Whole Blood Immunohematology Review 421
Skin preparation
Volume of blood routinely collected
Maximum volume
Low-volume collections
Volumes of anticoagulant
Time of collection
Samples for testing
Storage temperature of unit between
collection & processing
Aseptic method, e.g., povidone-iodine scrub & prep solution.450 mL ± 10% or 500 mL ± 10%, depending on collection bag.10.5 mL of blood per kg of donor’s weight, including samples for testing (e.g., 473 mL
from 100 lb [45 kg] donor).300–404 mL in 450-mL bag or 333–449 mL in 500-mL bag. Labeled “low-volume.” RBCs
may be transfused, but other components shouldn’t be prepared because of abnormal
anticoagulant-to-plasma ratio.63 mL anticoagulant for 450-mL collection, 70 mL for 500-mL collection.Usually <10 min. If >15–20 min, unit may not be suitable for preparation of platelets or
plasma.From diversion pouch or by 2nd phlebotomy.20 °–24°C if platelets are to be prepared; otherwise 1°–6°C.