Quick Review Cards for Medical Laboratory Science

Blood Components—RBCs Immunohematology Review 427

STORAGE

COMPONENT PREPARATION TEMPERATURE SHELF LIFE INDICATIONS OTHER

RBCs

RBCs adenine,
saline added

RBCs frozen

Should have HCT£80%;

otherwise not enough

preservative to support

RBCs. 1 unit should ↑

HGB 1 g/dL or HCT 3%.

Most commonly used

RBC product.

Osmolality to monitor

glycerol removal. Virtually

all plasma, anticoagu-

lant, WBCs, & platelets

removed. Safe for IgA-

deficient pt. Used to

store rare cells.

Separated from WB

by centrifugation or

sedimentation any

time before expira-

tion date of WB, or

collected by

apheresis.

Additive solution

added to RBCs fol-

lowing removal of

most plasma.

Frozen in glycerol

within 6 days of

collection. High

glycerol (40%)

method most

commonly used.

1 °–6°C

1 °–6°C

Frozen in high

glycerol: £ 65 °C.

After deglyc

(washing in ↓

concentration of

saline): 1°–6°C

35 days in CPDA-1

42 days

Frozen: 10 yr.

After degylc: 24 hr

(unless closed

system used)

Inadequate tissue

oxygenation

Same

Same

continued...