Grading Reactions—Tube Versus Gel Immunohematology Review 438
TUBE* GEL
4+
3+
2+
1+
Mixed field
Neg
*In tube testing, hemolysis is also a pos rxn.
Solid band of agglutinated RBCs at top
Band of agglutinated RBCs near top with a few staggered below
Agglutinates throughout
Agglutinates predominantly in lower half of column with some
RBCs at bottom
Layer of agglutinated RBCs at top & pellet of unagglutinated RBCs
at bottom
Well-defined pellet of unagglutinated RBCs at bottom
One solid agglutinate
Several large agglutinates
Medium-sized agglutinates, clear background
Small agglutinates, turbid background
Some agglutinated RBCs in sea of free RBCs
No agglutinates