Rh Typing Sera Immunohematology Review 450
HIGH-PROTEIN ANTI-D LOW-PROTEIN ANTI-D
Source
Control
Comments
Mixture of monoclonal IgM & monoclonal or polyclonal IgG.
Any neg typing rxn serves as control, e.g., neg rxn with anti-A or
anti-B. When RBCs react with all antisera (i.e., AB pos), run control
recommended by manufacturer. (Usually pt RBCs with autologous
serum or 6% or 8% albumin.)
Most widely used. Lower rate of false pos with Ig-coated RBCs.
Prepared from pools of human sera
(immunized Rh-neg individuals).
Same ingredients as reagent, except no
anti-D. Should be purchased from same
manufacturer as anti-D.
More false-pos than low-protein reagents,
e.g., RBCs with pos DAT.
