Photometric Methodscontinued Clinical Chemistry Review 74
METHOD PRINCIPLE COMPONENT PARTS OTHER
Chemiluminescence
Turbidimetry
Nephelometry
Chemical rxn that produces light.
Usually involves oxidation of
luminol, acridinium
esters, or dioxetanes.
Measures reduction in light
transmission by particles in
suspension.
Similar to turbidity, but light is
measured at angle from light
source.
Reagent probes, sample & reagent
cuvette, photomultiplier tube,
readout device
Light source, lens, cuvette,
photodetector, readout device
Light source, collimator, mono-
chromator, cuvette, photodetector,
readout device
Doesn’t require excitation radia-
tion or monochromators like
fluorometry. Extremely sensitive.
Used for immunoassays.
Used to measure proteins in
urine & CSF.
Used to measure ag-ab rxn.
