Science - USA (2022-04-08)

The rs4987360 association replicates in bulk RNA-seq eQTL data from eQTL-Gen ( 34 ) and GTEx ( 5 ) and has allelic effects with ...

rs3087243 but is in linkage equilibrium (R^2 = 0.5). rs231770-T is similarly associated with decreasedCTLA4mRNA expression in CD ...

genotype and cell-type proportion. At a study- wide significance threshold (p<3.0×10−^6 ), we identified five associations, a ...

onERN1expression in natural killer (NK) cells. Yet this same variant has a trans effect on SEC61GandSEC61Bbut not on the other g ...

on gene expression and identified more than 26,000 independent cis-acting eQTLs and 990 trans-eQTLs outside the MHC locus. Summa ...

causal loci are shared between an eQTL and GWAS risk loci. This observation can be ex- plained by either a causal effect or plei ...

were mapped to GRCh37/hg19 (release 84) using STAR ( 77 ). Cells were assigned using genotype data to individual participants us ...

Y.-F. Wanget al., Identification of 38 novel loci for systemic lupus erythematosus and genetic heterogeneity between ancestral ...

RESEARCH ARTICLE SUMMARY ◥ PROTEIN ENGINEERING Tuning T cell receptor sensitivity through catch bond engineering Xiang Zhao, Eli ...

RESEARCH ARTICLE ◥ PROTEIN ENGINEERING Tuning T cell receptor sensitivity through catch bond engineering Xiang Zhao^1 , Elizabet ...

toodistantfromthepMHCtoformdirect contacts in the bound state to mitigate se- lecting for affinity-matured TCRs. On the basis of ...

B35-Q65 and TCR55a-A98H (fig. S3H). BFP showed that B35-Q65A–HIV formed catch bonds with TCR55a-A98H but exhibited shorter peak ...

and a CDR2 mutation, TCR55b-A50D. We iden- tified the isolated TCR55b-A50D mutation as necessary and sufficient to enable T cell ...

a weak correlation between EC 50 and affinity (R^2 = 0.5998) (fig. S11C). We tested whether the eight high-sensitivity mutants s ...

HLA-A1–MAGE-A3+colon cancer cell line HCT-116 (Fig. 5, F to J, and fig. S15). In re- sponse to A375 cells, the engineered TCRs 9 ...

TCRs were cocultured with MAGE-A3 or TITIN peptide–pulsed antigen-presenting cells. Although 20a-18 or 94a-14 showed enhanced cy ...

to a yeast-display pMHC library system orig- inally used to characterize the cross-reactivity of TCRs ( 26 , 27 ) and to uncover ...

maintaining physiologic affinity, reducing the predisposition toward off-target cross-reactivity compared with affinity-matured ...

Pen-Strep (Thermo Fisher Scientific) at 37°C and 5% CO 2. LentiX cells and 293T cells were cultured in DMEM (Thermo Fisher Scien ...

once to remove excess peptides. MAGE-A3– specific TCR mutants-transduced SKW3 cells were resuspended at 5 × 10^5 cells/mL and 20 ...