Cell - 8 September 2016

STAR+METHODS

KEY RESOURCES TABLE

REAGENT or RESOURCE SOURCE IDENTIFIER
Chemicals, Peptides, and Recombinant Proteins

3-(Decyldimethylammonio) propanesulfonate inner salt Sigma Cat#D4266
Glutaraldehyde (EM Grade Distillation Purified, 25% solution) Electron Microscopy
Sciences

Cat#16220

1,1’-(suberoyldioxy)bisazabenzotriazole (SBAT) Bich et al., 2010 N/A

Formaldehyde Sigma Cat# F8775

Deposited Data
Full Med-PIC cryo-EM Map This paper EMD-8308

DTFIIH-E Med-PIC cryo-EM Map (Focused refinement) This paper EMD-8307
DTail Med-PIC cryo-EM Map (Focused refinement) This paper EMD-8305
Med-PIC model (DTail Med-PIC fit) This paper PDB: 5SVA

Raw MS-MS data This paper massIVE: MSV000080013
(http://massive.ucsd.edu)
Crosslinking-MS annotated spectra: Med-PIC Search
Key = an9t4zxaa7

This paper http://prospector2.ucsf.edu/prospector/cgi-bin/
msform.cgi?form=msviewer
Crosslinking-MS annotated spectra: Med+PolII Search
Key = oiba4ekrvi

This paper http://prospector2.ucsf.edu/prospector/cgi-bin/

msform.cgi?form=msviewer

Experimental Models: Organisms/Strains
S. cerevisiae: N-TAP MED17 and C-ProtG RPB1 CB010 (Mata
pep4::HIS3 prb1::LEU2 prc1::HISG can1 ade2 trp1 ura3 his3
leu2–3,112cir-oGAL+ RAF+ SUC+ RPB1::3C-PROTG::Kl.URA3
MED17p::loxP::TAP-MED17)

Robinson et al., 2012 N/A

S. cerevisiae: C-TAP MED8 andDMED16 CB010 (Matapep4::HIS3
prb1::LEU2 prc1::HISG can1 ade2 trp1 ura3 his3 leu2–3,112cir-o
GAL+ RAF+ SUC+ med16::kanMX6 MED8::TAP::Kl.TRP1)

Robinson et al., 2012 N/A

S. cerevisiae: C-TAP TFB3 CB010 (Matapep4::HIS3 prb1::LEU2
prc1::HISG can1 ade2 trp1 ura3 his3 leu2–3,112 cir-o GAL+ RAF+
SUC+ tfb6::kanMX6 TFB3::TAP::Kl.TRP1)

Murakami et al., 2012 N/A

S. cerevisiae: C-TAP TFG2 CB010 (Matapep4::HIS3
prb1::LEU2 prc1::HISG can1 ade2 trp1 ura3 his3 leu2–3,112
cir-o GAL+ RAF+ SUC+ TFG2::TAP::Kl.TRP1)

Murakami et al., 2012 N/A

S. cerevisiae: C-TAP MED5 + C-TAP mutCTD RPB1 CB010
(Matapep4::HIS3 prb1::LEU2 prc1::HISG can1 ade2 trp1 ura3
his3 leu2–3,112 cir-o GAL+ RAF+ SUC+ MED5::TAP::Kl.TRP1,
contains plasmid [pRS316rpb1::3CYS-1ARG CTD])

This paper N/A

S. cerevisiae: RPB1-1461TEV-ProtG S288C (his3D200 leu2D 0
or 1 ura3-52 lys2-128dtrp1D63 rpb1D::CLONATMX,contains
plasmid [pRS315rpb1::1461TEV::ProtG])

This paper (derived

from CKY749 C. Kaplan

laboratory)

N/A

S. cerevisiae: C-ProtA RPB1 andDRPB4 CB010 (Matapep4::HIS3
prb1::LEU2 prc1::HISG can1 ade2 trp1 ura3 his3 leu2–3,112 cir-o
GAL+ RAF+ SUC+ rpb4::Kl.URA3 RPB1::TEV-PROTA::Kl.TRP1)

Liu et al., 2010 N/A

Recombinant DNA
Plasmid: Full-LengthS.c TBP(pRSFDuet) Murakami et al., 2012 N/A
Plasmid: Full-LengthS.c Toa1(pRSFDuet) Murakami et al., 2012 N/A
Plasmid: Full-LengthS.c Toa2(pRSFDuet) Murakami et al., 2012 N/A

Plasmid: Full-LengthS.c TFIIB(pTYB2) Liu et al., 2010 N/A
Plasmid: Full-LengthS.c Tfa1(MCSI) andTfa2(MCSII) (pRSFDuet) Murakami et al., 2012 N/A
Plasmid: Full-LengthS.c TFIIS(pRSFDuet) Murakami et al., 2013 N/A

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Cell 166 , 1411–1422.e1–e8, September 8, 2016 e1