Cell - 8 September 2016

CONTACT FOR REAGENT AND RESOURCE SHARING

Further information and requests for reagents may be directed to the corresponding author Roger Kornberg (kornberg@stanford.
edu).

Continued

REAGENT or RESOURCE SOURCE IDENTIFIER
Plasmid: Full-LengthS.c Gcn4(pET30a) Robinson et al., 2012 N/A
Plasmid:His4promoter fragment [-92/+16] (pUC19) This paper N/A

Plasmid: 3Cys:1Arg mut.S.cCTD
3C- partial ProtG
fragment (pUC57)

This paper N/A

Plasmid: 3Cys:1Arg mut.S.cCTD
3C- ProtG fragment
(pCeMM-CTAP(SG))

This paper N/A

Plasmid:RPB1p::rpb1::3CYS-1ARG CTD::3C::ProtG(pRS316) This paper (derived
from pCK518 C. Kaplan
laboratory)

N/A

Plasmid:RPB1p::rpb1:1461TEV::3C::ProtG(pRS315) This paper (derived
from pCK858 C. Kaplan
laboratory)

N/A

Sequence-Based Reagents
Primer: C-terminalRPB1-3C-Protein GForward: 5^0 -GGAGGATTT
ACAGCGTACGGTGGTGC-3^0

This paper N/A

Primer: C-terminalRPB1-3C-Protein GReverse: 5^0 -TGCTATTAC
GTACTATTCAGTGACAGTGAAAGTCTTTGTCGCGTC
30

This paper N/A

Software and Algorithms
UCSF tomography Zheng et al., 2007 http://www.msg.ucsf.edu/Tomography/
tomography_main.html
IMOD version 4.5.2 Kremer et al., 1996 http://bio3d.colorado.edu/imod/;
RRID: SCR_003297

BSOFT version 1.6.3 Heymann et al., 2008 http://lsbr.niams.nih.gov/bsoft/
Relion version 1.4 Scheres, 2012 http://www2.mrc-lmb.cam.ac.uk/relion/
index.php/Main_Page
EMAN2 version 2.1 Tang et al., 2007 http://blake.bcm.edu/emanwiki/EMAN2

Proteome Discoverer 1.4 Thermo Scientific https://www.thermofisher.com/order/catalog/
product/IQLAAEGABSFAKJMAUH;
RRID: SCR_014477
Protein Prospector 5.14.4 Trnka et al., 2014 http://prospector.ucsf.edu/prospector/
mshome.htm; RRID: SCR_014558
ProteOn Manager version 3.1.0.6 Bio-Rad http://www.bio-rad.com/en-us/product/
proteon-manager-software

Biacore T200 Evaluation Software version 1.0 GE Healthcare https://www.biacore.com/lifesciences/
UCSF Chimera 1.10.2 Pettersen et al., 2004 https://www.cgl.ucsf.edu/chimera/;
RRID: SCR_004097
Coot version 0.8.3 Emsley et al., 2010 http://www2.mrc-lmb.cam.ac.uk/personal/
pemsley/coot/; RRID: SCR_014222
Python Modeling Interface (PMI library) version be72c15 Andre Sali Lab https://github.com/salilab/pmi

Integrative Modeling Platform version 829c3f0 Andre Sali Lab http://salilab.org/imp; RRID: SCR_002982

Other
MonoCap C18 HighResolution 0.1 mm id x 2000 mm monolithic
silica capillary HPLC column

GL Sciences Cat#5020-10015

Superdex Peptide 3.2/300 GE Life Sciences Cat#29-0362-31

e2 Cell 166 , 1411–1422.e1–e8, September 8, 2016