Cell - 8 September 2016

Article

A Genome-wide CRISPR Screen in Toxoplasma

Identifies Essential Apicomplexan Genes

Graphical Abstract

Highlights

d CRISPR enables the first genome-wide loss-of-function

screen inT. gondii

d The screen measures each gene’s impact during infection of

human fibroblasts

d Functional assays validate the essentiality of formerly

uncharacterized proteins

d An invasion factor found in all apicomplexans is essential in

malarial parasites

Authors

Saima M. Sidik, Diego Huet,

SureshM. Ganesan, ...,Vern B. Carruthers,

Jacquin C. Niles, Sebastian Lourido

Correspondence

[email protected]

In Brief

The first genome-wide genetic screen of

an apicomplexan parasite identifies an

invasion factor essential for the fitness of

all parasites in this phylum, including

those causing malaria and toxoplasmosis.

Sidik et al., 2016, Cell 167 , 1423–1435

September 8, 2016ª2016 Elsevier Inc.

http://dx.doi.org/10.1016/j.cell.2016.08.019