Cell - 8 September 2016

Article

Sequential Immunization Elicits Broadly Neutralizing

Anti-HIV-1 Antibodies in Ig Knockin Mice

Graphical Abstract

Highlights

d Immunization elicits anti-HIV-1 broadly neutralizing

antibodies in knockin mice

d ELISA-guided sequential immunization induces high levels

of somatic mutation

d Mouse antibodies elicited by sequential immunization

resemble human antibodies

Authors

Amelia Escolano, Jon M. Steichen,

Pia Dosenovic, ..., Dennis R. Burton,

William R. Schief, Michel C. Nussenzweig

Correspondence

[email protected] (W.R.S.),

[email protected] (M.C.N.)

In Brief

Generation of HIV broadly neutralizing

antibodies—a major goal in vaccine

research—can be achieved through

sequential immunization with HIV

envelope glycoproteins designed to

engage PGT121 antibody precursors and

their intermediates.

Escolano et al., 2016, Cell 166 , 1445–1458

September 8, 2016ª2016 Elsevier Inc.

http://dx.doi.org/10.1016/j.cell.2016.07.030