STAR+METHODS
KEY RESOURCES TABLE
REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies
Peroxidase-conjugated AffiniPure Goat Anti-mouse IgG Jackson laboratories Cat#115-035-071 RRID: AB_2338506
Peroxidase-conjugated AffiniPure Goat Anti-human IgG Jackson laboratories RRID: AB_2337532
Rb pAb to 6X His tag Abcam Cat#ab9108 RRID: AB_307016
Anti-Mouse Ly-6G (Gr1) APC-eFluor780 clone RB6-8C5 eBioscience Cat#47-5931-82, RRID: AB_1518804
Anti-Mouse F4/80 APC-eFluor 780 clone BM8 eBioscience Cat#47-4801-82 RRID: AB_1548745
Anti-Mouse CD8a APC-eFluor 780 clone 53-6.7 eBioscience Cat#47-0081-82 RRID: AB_1272185
Anti-Mouse CD4 APC-eFluor 780 clone RM4-5 eBioscience Cat#47-0042-82 RRID: AB_1272183
Anti-Hu/Mo CD45R (B220) FITC clone RA3-6B2 eBioscience Cat#11-0452-85 RRID: AB_465055
Anti-Mouse CD38 Alexa Fluor 700 clone 90 eBioscience Cat#56-0381-82 RRID: AB_657740
Anti-Mouse IgM PerCP-eFluor 710 clone II/41 eBioscience Cat#46-5790-82 RRID: AB_1834435
Anti-Mouse CD21/CD35 eFluor 450 clone eBio4E3 eBioscience Cat#48-0212-82 RRID: AB_2016703
BV421 rat Anti-Mouse IgG1 clone A85-1 BD Biosciences Cat#562580
PE anti-mouse CD23 clone B3B4 BioLegend Cat#101607 RRID: AB_312832
PE/Cy7 anti-mouse IgD clone 11-26c2a BioLegend Cat#405720 RRID: AB_2561876
PE anti-mouse Ig light chainlclone RML-42 BioLegend Cat#407308 RRID: AB_1027659
Chemicals, Peptides, and Recombinant Proteins
CD43 (Ly-48) Microbeads, mouse Miltenyi Biotec Cat#130-049-801
Sigma Adjuvant System Sigma Cat#S6322-1VL
ABTS Single solution (Ready to use) Life technologies Cat#00-2024
Imject Alum Thermo scientific Cat#77161
Comp Beads Negative control BD Biosciences Cat#51-90-9001291
Comp beads anti-Rat and anti-Hamster Ig, K BD Biosciences Cat#51-90-9000949
ArC negative beads Life technologies Cat#A10346
ArC reactive beads Life technologies Cat#A10346
APC Streptavidin BioLegend Cat#405207
PE Streptavidin BioLegend Cat#405203
RNAsin RNase inhibitor Promega Cat#N2615
Nuclease-free water QIAGEN Cat#129114
Superscript III Invitrogen Cat#18080-044
HotStarTaq DNA Polymerase Qiagen Cat#203203
Protein G Sepharose 4 Fast Flow GE Healthcare Cat#17-0618-05
Polyethylenimine, branched Sigma Cat#408727-100ML
Tween 20 Sigma Cat#P7949
11MUTA Steichen et al., 2016 N/A
10MUT Steichen et al., 2016 N/A
7MUT Steichen et al., 2016 N/A
5MUT Steichen et al., 2016 N/A
BG505 SOSIP T332N (VLC2) Steichen et al., 2016 N/A
VLC1 Steichen et al., 2016 N/A
VLC3 Steichen et al., 2016 N/A
VLC4 Steichen et al., 2016 N/A
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