Figure S2. Priming of GLHL121 Knockin Mice, Related toFigure 1
(A) Table shows a list of priming strategies tested in GLHL121 knockin mice. The name of the immunogen, the adjuvant and the number of immunizations are
specified.
(B) ELISA analysis of the serum collected after immunization of GLHL121 knockin mice as indicated in (A). Binding to the protein indicated is represented in blue for
immunized mice and in red for naive mice. The binding of the human PGT121 bNAb is shown in black (1/100 starting dilution of a 500mg/ml solution = 5mg/ml).
(C) Binding affinities of the human PGT121, the MutHGLL121 and the GLHL121 monoclonal antibodies to 10MUT as determined by Surface Plasmon Resonance
(SPR).
(D) Neutralization activity in the serum of GLHL121 and MutHGLL121 knockin mice after immunization with 10MUT. The serum (1/50 dilution) of 3 GLHL121 and 1
MutHGLL121 mice was tested in a TZM-bl assay against 3 Tier 2/1B HIV-1 isolates and Murine Leukemia Virus (MuLV) as a negative control. ND = not detectable.
(E) Diagram showing the sequential immunization protocol.
(F) ELISA analysis of the serum collected after immunization of GLHL121 knockin mice as specified in (E). Graphs show the binding of mouse serum to the different
proteins at the different steps of the immunization protocol.
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