Cell - 8 September 2016

Figure S5. Somatic Mutations in Immunized GLHL121 and MutHGLL121 Mice, Related toFigures 4and 5
(A and B) (A) Diagram of the immunization protocol (B) Number of amino acid mutations in the IGK of single antigen specific B cells isolated from sequentially
immunized GLHL121 (mice M1-M7) (left) and MutHGLL121 (mice M8-M14) (right) mice. Each column represents one mouse and each dot one B cell.
(C) Number of amino acid mutations in the IGH of single antigen specific B cells isolated from sequentially immunized GLHL121 (mice M1-M7) (left) and MutH
GLL121 (mice M8-M14) (right) mice. Each column represents one mouse and each dot one B cell.
(D) Number of amino acid mutations in the IGK from sequentially immunized GLHL121 and MutHGLL121 mice. Pooled sequences from the 7 mice of each ge-
notype. Each dot represents one sequence from a single antigen specific B cell. ns = not statistically significant.
(E) Number of amino acid mutations in the IGH from sequentially immunized GLHL121 and MutHGLL121 mice. Pooled sequences from the 7 mice of each ge-
notype. Each dot represents one sequence from a single antigen specific B cell. p < 0.0001.
(F) Tables show the number and position of RGYW/WRCY AID targeting sites in GLH121 (top) and MutH121 (bottom).
(G) Number of amino acid mutations in the IGK (top left) and IGH (bottom left) of 3 GLHLmice (mice M15-M17) and number of nucleotide mutations in the IGK (top
right) and IGH (bottom right) of 2 MutHGLLmice (mice M23 and M25) immunized as indicated in the diagram above. Each column represents one mouse and each
dot one antigen specific B cell.
(H) Number of nucleotide mutations in the IGK (top) and IGH (bottom) of MutHGLLmice immunized according to Protocol 1 or Protocol 2 detailed in (A) and (G)
respectively. p < 0.0001.
(I) Number of nucleotide (left) and amino acid (right) mutations in the IGK (top) and IGH (bottom) of 2 GLHL121 mice (mice M27-M28) immunized as indicated in the
diagram above. Each column represents one mouse and each dot one antigen specific B cell.