Cell - 8 September 2016

Figure S6. ELISA for Monoclonal Antibodies from Sequentially Immunized Knockin Mice against Engineered and Native-like Proteins,
Related toFigures 6and 7
(A) Binding of 3 HIV-1 neutralizing antibodies cloned from a sequentially immunized GLHL121 knockin mouse to the wtBG505 SOSIP (left) and 10MUT/10MUT KO
(right). Human PGT121 bNAb was included for comparison. Antibodies were tested at a starting concentration of 10mg/ml.
(B) Binding of 2 non-neutralizing antibodies cloned from sequentially immunized GLHL121 mice (Ab 18 and Ab19) to wtBG505 SOSIP in ELISA. The human
PGT121 bNAb was included for comparison. Antibodies were tested at a starting concentration of 10mg/ml.
(C) Table shows the results of TZM-bl assays performed against a panel of Tier2/1B isolates with antibodies cloned from MutHGLLmice immunized according to
Protocol 1 or Protocol 2.