STAR+METHODS
KEY RESOURCES TABLE
CONTACT FOR REAGENT AND RESOURCE SHARING
Further information and requests for reagents may be directed to, and will be fulfilled by the corresponding author William R. Schief
([email protected]).
REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies
anti-GL7 PerCP-eFluor710 eBioSciences Cat#:46-5902-82
anti-CD38 PE-Cy7 eBioSciences Cat#:25-0381-82
anti-CD19 BUV396 Beckton Dickinson Cat#:563557
anti-B220 BUV396 Beckton Dickinson Cat#:563793
anti-CD8 APC-Cy7 Beckton Dickinson Cat#:560182
anti-CD4 APC-Cy7 Beckton Dickinson Cat#:560181
anti-F4/80 APC-Cy7 BioLegend Cat#:123118
anti-Gr-1 APC-Cy7 Beckton Dickinson Cat#:557661
anti-CD11c APC-Cy7 Beckton Dickinson Cat#:561241
anti-IgM BV786 Beckton Dickinson Cat#:564028
anti-IgD BV605 Beckton Dickinson Cat#:563003
Chemicals, Peptides, and Recombinant Proteins
eOD-GT8 60mer Jardine et al., 2015; 2016a N/A
BG505 core-GT3 nanoparticles This paper N/A
BG505 SOSIP-GT3 This paper N/A
BG505 SOSIP N276D This paper N/A
eOD-GT8 Jardine et al., 2015; 2016a N/A
eOD-GT8 KO Jardine et al., 2015; 2016a N/A
r1-core-N276D This paper N/A
r1-core-KO This paper N/A
ABC SOSIP N276D cocktail This paper N/A
Deposited Data
Antibody sequence data and analysis
scripts
This paper https://github.com/briney/VRC01gH-GT3;
GenBank: KX779470–KX779519 and
KX808478–KX808481Experimental Models: Cell Lines
TZM-bl cells NIH AIDS reagent program Cat#:8129
HEK293S GnTI- cells ATCC Cat#:CRL-3022
Experimental Models: Organisms/Strains
VRC01 gH mouse Jardine et al., 2015 N/A
Sequence-Based Reagents
Murine PCR primers Tiller et al., 2008;Table S4 N/A
Human VH1-2 PCR primer Sok et al., 2014;Table S4 N/A
Software and Algorithms
AbStar https://www.github.com/briney/abstar
Clonify Briney et al., 2016 https://www.github.com/briney/clonify
Sickle https://www.github.com/najoshi/sickle
Cutadapt Martin, 2011 https://github.com/marcelm/cutadapt
PANDAseq Masella et al., 2012 https://github.com/neufeld/pandaseq
Cell 166 , 1459–1470.e1–e5, September 8, 2016 e1