Cell - 8 September 2016

Figure S4. Sequence and Antigenicity of the r1-Core Resurfaced Core gp120, Related toFigure 2
(A) Sequence alignment of r1-core with gp120core-e-2CC HxB2. The r1-core is derived from the core-e-2CC HxB2 protein that we previously described (Jardine
et al., 2015) and is also known as gp120core-e-2CC_HxB2_r1. A total of 78 surface positions have been modified, out of 358 total residues. Mutations are
classified in the third row of the alignment, with ‘‘’’: denoting a conservative mutation between amino acids with similar physicochemical properties; ’’ ’’ denoting a
non-conservative mutation; and ‘‘.’’ denoting a semi-conservative mutation.
(B) Dissociation constants for core-e-2CC HxB2, r1-core, and RSC3 (Wu et al., 2010) with VRC01-class bnAbs. (C) ELISA binding of mature VRC01 to core-
N276D (core-e-2CC HxB2 with the N276D mutation; inverted triangles), r1-core-N276D (circles), r1-core (squares) and r1-core-KO (triangles). Starting con-
centration of mature VRC01 was 2mg/ml.