Cell - 8 September 2016

Article

Induction of HIV Neutralizing Antibody Lineages in

Mice with Diverse Precursor Repertoires

Graphical Abstract

Highlights

d Mice generate diverse primary B cell receptors from a single

human VHsegment

d An efficient new method generated mouse models for

assessing human vaccination strategies

d Step-wise immunization induced affinity maturation of HIV-

neutralizing antibodies in mice

Authors

Ming Tian, Cheng Cheng, Xuejun Chen, ...,

Barton F. Haynes, John R. Mascola,

Frederick W. Alt

Correspondence

[email protected] (J.R.M.),

[email protected] (F.W.A.)

In Brief

Sequential immunization using modified

HIV envelope glycoproteins supports the

maturation of HIV-neutralizing antibody

lineages in mouse models that have

diverse antibody repertoire and therefore

are closer to physiological immunization

settings than conventional transgenic

models.

Tian et al., 2016, Cell 166 , 1471–1484

September 8, 2016ª2016 Elsevier Inc.

http://dx.doi.org/10.1016/j.cell.2016.07.029