Figure 1.gdT Cells Are Ubiquitous and Activated in Human PDA
(A) Frozen sections of human PDA and normal pancreas were stained using a mAb specific for TCRg/dor isotype control. Representative images and quantitative
data are shown.
(B) Single-cell suspensions from human PDA tumors and PBMCs were co-stained for CD45, CD3, and TCRg/d. The percentage ofgdT cells among CD3+cells
was calculated. Representative contour plots and summary data are shown. Each dot represents a different patient sample.
(C) The percentage of PDA-infiltratinggdT cells among CD45+cells was compared with tumor-infiltrating cells expressing select myeloid differentiation markers.
(D) The percentage of PDA-infiltrating and PBMCgdT cells among CD3+cells was compared with that of CD4+and CD8+abT cell subsets in each respective
compartment.
(E) PBMC and PDA-infiltrating CD3+TCRg/d+cells from PDA patients were gated and co-stained using mAbs specific for CD45RA and CD27. The gating par-
adigms for Tnaive,TCM,TEM, and TEM-RApopulations are shown. Representative contour plots and quantitative data indicating the fraction of TEMgdT cells in each
compartment are indicated.
(F and G) PDA-infiltrating and PBMCgdT cells from PDA patients were stained using mAbs specific for CD62L (F) and Vg9 (G). Representative histograms
and quantitative data are shown. Human data are based on tumor tissue or PBMCs analyzed from nine to 13 PDA patients (p < 0.05, p < 0.01,
p < 0.001).
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